Choosing Between qPCR vs. dPCR vs. ddPCR for Robust Analytics & Regulatory Compliance
Time: 9:00 am
day: Pre-Conference QC Readiness Track B - AM
Details:
In gene therapy development, accurate quantification of viral vectors is critical for assessing potency and ensuring therapeutic efficacy. This discussion will explore the strengths and limitations of qPCR, dPCR, and ddPCR, and how selecting the right method impacts early versus late-stage gene therapy processes.
- PCR Method Comparison Pros & Cons: Overview of qPCR, dPCR, and ddPCR for gene therapy, focusing on their use in viral vector quantification and potency testing
- Early vs. Late-Stage Assay Selection: Why early-stage programmes favour dPCR/ddPCR, while late-stage programmes often rely on qPCR due to established workflows
- Pitfalls of qPCR in Late-Stage Gene Therapy: Challenges of using qPCR for AAV titration and potency in late-stage programmes, and how dPCR/ddPCR can improve accuracy and regulatory compliance
