Kamila Pytel
Associate Director Analytical Development Avantgarde
Seminars
Wednesday 6th May 2026
Challenges & Solutions in Developing Potency Assays for AAV Vectors
10:15 am
- Understanding vector design, transgene sequence and delivery target in the aspect of potency assay design
- Protein expression vs gene expression vs dose-response cell-based potency assays: what is needed for AAV vector characterisation/release and why?
- Development of in vivo potency assay versus in vitro potency assay for AAV vectors: at what stages of product development is in vitro testing desirable, and can one replace the other?
- Importance of selecting the product specific cell line and developing optimised, reproducible transductions in moving towards a consistent product specific potency method
