Steven Wolk

Steven Wolk

Company: Editas

Job title: Vice President, Analytical Chemistry & Site Head Boulder


Characterization of gRNAs and Ribonucleoproteins for CRISPR Applications 12:00 pm

gRNA sequences can form alternate intra-molecular and intermolecular folds, which can impact complexation with the Cas protein Formation is sequence dependent, and more likely for longer sequences Manifestation also is condition dependent RNPs represent another level of complexity due to noncovalent nature of complex SEC, IEX, PAGE, IM data shows that the secondary structures formed…Read more

day: Track B Day 2

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