Characterization of gRNAs and Ribonucleoproteins for CRISPR Applications

Time: 12:00 pm
day: Track B Day 2


  • gRNA sequences can form alternate intra-molecular and intermolecular folds, which can impact complexation with the Cas protein Formation is sequence dependent, and more likely for longer sequences
  • Manifestation also is condition dependent
  • RNPs represent another level of complexity due to noncovalent nature of complex
  • SEC, IEX, PAGE, IM data shows that the secondary structures formed by the guides impact the complexation
  •  Some method dependence of results, which is not surprising for these noncovalent complexes of large biomolecules